Synergistic Influences of Essential Oils Blends and Their Active Substances on Blood Biochemical Constituents Under Climate Change of Growing Saidi Lambs.

Abdelfarrag, Mahmoud M.; Khattab, Hamdy M.; Khorshed, Mahmoud Mohamed; Singer, Abdalla Mansour

doi:10.21608/aujst.2025.435513

Synergistic Influences of Essential Oils Blends and Their Active Substances on Blood Biochemical Constituents Under Climate Change of Growing Saidi Lambs.

Document Type : Original papers

Authors

¹ Animal and Poultry Production Department, Faculty of Agriculture and Natural Resources, Aswan University, Egypt

² Department of Animal Production, Faculty of Agriculture, Ain Shams University, Egypt.

³ Animal Production,Faculty of Agriculture,Ain Shams university,Egypt.

⁴ Faculty of Agriculture and Natural Resources, Aswan University, Department of Animal and poultry production

10.21608/aujst.2025.435513

Abstract

This study investigated the synergistic effect of two blends of essential oils from medicinal plants as a phytogenic additive on the active substances and on various blood serum parameters of Saidi lambs. The initial additive comprised a 1:1:1 Rosemary, Thyme, and Peppermint oil mixture (RTPOM). The second additive comprised a 1:1:1 Lemongrass, Marjoram, and Black Seed oil mixture (LMBOM). The two groups were orally given to Saidi lambs by syringe. The 1^st group (5 lambs) was administered a control diet devoid of essential oil blends supply (concentrate feed mixture to roughage ratio of 70:30); the 2^nd group (5 lambs) was administered a control diet supplemented with 0.03% RTPOM on a DM basis; and the 3^rd group administered a control diet supplemented with 0.03% LMBOM on a DM basis. The experiment spanned 180 days. GC-MS analyzed all essential oils of medicinal plants for their bioactive substances. Results revealed that the principal bioactive substances for RTPOM were eucalyptol and thymol for Rosemary; thymol and p-Cymene for Thyme; and (-)-carvone and limonene for Peppermint. The principal bioactive substances for LMBOM were citral and 1,3,8-p-menthatriene for Lemongrass; terpinen-4-ol and γ-terpinene for Marjoram; and heptane, 2,2,4,6,6-pentamethyl-, thymoquinone, and benzene,1-methyl-3-(1-methylethyl)- for Black Seed. A substantial rise in serum total protein (P=0.0069) and globulin (P=0.0453) and a decrease in cholesterol (P=0.0429) were seen with the LMBOM and RTPOM rations. Furthermore, the incorporation of essential oil blends led to a significant reduction in rectal temperature (P<0.0001) in a hot and cool environment compared to the lambs in control.

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