Bacterial Endophytes from Hibiscus sabdariffa Roots: A Source of Growth-Promoting Abilities Candidates.

Ibrahim, Zohor Ahmed; Soliman, Wagdi Saber; Konswoa, Osama Ahmed; Abbas, Mohammed Tawfik

doi:10.21608/aujst.2025.398967.1192

Bacterial Endophytes from Hibiscus sabdariffa Roots: A Source of Growth-Promoting Abilities Candidates.

Document Type : Original papers

Authors

¹ Department of microbiology, Faculty of agriculture and natural resources, Aswan university, Aswan, Egypt

² Horticulture Department, Faculty of Agriculture and Natural Resources, Aswan University

³ Department of Biochemistry, Faculty of Agriculture, Cairo University.

⁴ Agricultural Microbiology, Faculty of Agriculture and Natural Resources, Aswan University, Aswan, Egypt

10.21608/aujst.2025.398967.1192

Abstract

This study aimed to isolate and characterize native strains from the endophytic rhizospheric of (Hibiscus sabdariffa L.) plants to evaluate their potential as plant growth-promoting rhizobacteria(PGPRs). A total of 32 bacterial isolates were obtained and subjected to various biochemical tests, as well as assessments of plant growth-promoting traits such as phosphate and potassium solubilization, indole acetic production, nitrogen fixation assay and evaluating its capacity to endure varying temperatures as a measure of stress. The results showed that the inner shell of roselle plants roots in Aswan hosts a diverse group of microbes, as HER18Disolates were able to produce the highest value of indole among 32 isolates, reaching (38.87 mgl-1), while isolate HER9D excelled in nitrogen fixation, reaching (43.87mgl-1).HER35D and HER13D were the highest in the (SI), (PSI) and (KSI), respectively (2.54 and 3.63 cm)-1. It is interesting to note that most of the isolates excelled in tolerating high temperatures such as HER9D, HER20D, HER37D and HER25D. Some isolates also excelled in combining multiple functions of growth-promoting traits and stress resistance, such as HER18D.Based on PGP traits, different isolates were identified that are considered potent and can be used and applied as PGPRs for different plants and improving organic agriculture to achieve a sustainable environment.

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